Summary
This review examines studies on unintended genetic alterations caused by traditional and modern genetic modification techniques, including Agrobacterium-mediated transfer, biolistic bombardment, and CRISPR-Cas9 (delivered by various methods). Findings show that these techniques can produce a wide range of unintended genomic changes—from small mutations to major structural variations like chromosome truncations and large DNA rearrangements. Only a few studies used whole-genome or long-read sequencing, suggesting many such effects may be underestimated. The authors emphasize the need for improved analytical methods to better detect and assess these unintended modifications, helping regulators evaluate the safety of genetically modified and gene-edited organisms.